Cord Blood Processing

Our highly trained and dedicated laboratory staff members process each private and donated cord blood sample separately and employ state-of-the-art instrumentation for counting and separating nucleated cells.

  1. Cord blood is collected from the umbilical cord immediately after the birth of the baby and after the cord has been cut.  Bar coded labels are assigned and used on the cord blood collection bag and throughout the process so the cord blood stem cells can always be identified.
  2. Once the cord blood has been collected, the cord blood kit is picked up at the hospital by our courier and immediately delivered to our laboratory for processing.
  3. After the cord blood has been received and the identity confirmed, a cord blood sample is initially taken and evaluated to determine the number of stem cells in the cord blood.
  4. The cord blood is then processed by centrifugation to separate the white cells (where the stem cells are located) from the red blood cells and plasma. The processed cord blood stem cell unit is stored in a compartmented bag with small testing sections, which can be removed, thawed and tested if the unit is being considered for transplant.
  5. Samples are taken to evaluate several quality control parameters including viability, the number of CD34+ stem cells, bacterial and fungal contamination, and colony-forming assays.
  6. A cryopreservant is added to the cord blood stem cell freezing bag.  The freezing bag is gradually cooled in a control rate freezer to approximately -90oC and then transferred and stored in vapor phase liquid nitrogen freezer at -196oC for long-term storage.
  7. In the event where a cord blood stem cell unit is requested, the  Celebration Stem Cell centre staff will coordinate with your doctor or transplant specialist to arrange for retrieval of your cryogenically preserved cord blood stem cells.